Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA profiling of the dorsal root ganglia, spinal cord, and anterior cingulate cortex in mice with CFA-induced inflammatory pain.

Scientific reports·2026
Same author

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Knockout of TaCKX2.2 in Wheat Improved Grain Yield by Increasing Grain Number Per Spike and Grain Size Under Field Production Conditions.

Plant, cell & environment·2026
Same author

Post-Translational Control of TaFT1 by WAPO1 Ubiquitination Shapes Spike Architecture and Yield in Wheat.

Plant biotechnology journal·2026
Same author

Genomic and genetic dissection of drought tolerance in a resilient wheat germplasm JIN50.

Nature genetics·2026
Same author

Allelic variation in UVR8 modulates thermotolerance-yield tradeoffs in plants.

Cell research·2026
Same journal

Defined bacterial consortium highlights the impact of intestinal bacteria on DNA methylation and tumorigenesis.

Genome biology·2026
Same journal

Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana.

Genome biology·2026
Same journal

UK Biobank whole-genome sequencing reveals robust contributions of rare variants to complex-trait heritability.

Genome biology·2026
Same journal

A one-week automated genome-wide optical pooled screen using OttoSeq.

Genome biology·2026
Same journal

Integrated lipidomic and transcriptomic profiling of the host response in human malaria.

Genome biology·2026
Same journal

Centromeric satellite expansion drives genome evolution in the snowy owl.

Genome biology·2026
See all related articles

Related Experiment Video

Updated: Jul 25, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

11.7K

Efficient and versatile multiplex prime editing in hexaploid wheat.

Pei Ni1, Yidi Zhao1, Ximeng Zhou1

  • 1Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.

Genome Biology
|June 29, 2023
PubMed
Summary
This summary is machine-generated.

We enhanced prime editing efficiency in wheat using ePPEplus, achieving significant improvements. This breakthrough enables multiplex gene editing for stacking multiple agronomic traits in plants.

Keywords:
Hexaploid wheatMultiplex genome editingPrime editingReverse transcriptase

More Related Videos

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

5.9K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K

Related Experiment Videos

Last Updated: Jul 25, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

11.7K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

5.9K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K

Area of Science:

  • Plant biotechnology
  • Gene editing technologies
  • Crop improvement

Background:

  • Prime editing (PE) offers precise DNA modifications but faces efficiency limitations in plants.
  • Existing prime editing systems require optimization for broad application in crop species like wheat.

Purpose of the Study:

  • To develop an improved prime editing system for hexaploid wheat with enhanced efficiency.
  • To establish a multiplex prime editing platform for simultaneous multi-gene editing in wheat.

Main Methods:

  • Engineered an upgraded plant prime editor (ePPEplus) by introducing a V223A substitution into the reverse transcriptase of the ePPEmax architecture.
  • Tested ePPEplus in hexaploid wheat protoplasts and regenerated plants for efficiency and multiplexing capability.

Main Results:

  • ePPEplus demonstrated significantly higher editing efficiency, averaging 33.0-fold and 6.4-fold increases over previous systems.
  • Achieved robust multiplex prime editing, simultaneously editing 4-10 genes in protoplasts and up to 8 genes in regenerated plants.
  • Observed editing frequencies up to 74.5% in multiplex editing experiments.

Conclusions:

  • The ePPEplus system substantially enhances prime editing efficiency and capability in hexaploid wheat.
  • The developed multiplex prime editing platform is a powerful tool for stacking multiple agronomic traits in wheat.
  • This advancement broadens the application of prime editing for accelerating crop breeding and genetic improvement.