Utilization of non-pathogenic bacteria to obtain optimum biofilm production for beneficial applications

Depending on the bacteria embedded in the extracellular polymeric layer, biofilms can be advantageous or harmful. The isolated strains used in this investigation are already established to be beneficial biofilm-producing bacteria. In order to use them effectively in various domains, it is necessary to characterize them and understand their ideal physiological characteristics for maximum biofilm growth. This study used genome sequence analysis to identify and characterize strains isolated from water samples in Raipur, Chhattisgarh, India. The nucleotide sequences were submitted to NCBI GenBank under the accession numbers Bacillus tequilensis (MN889418) and Pseudomonas beteli (MN889419) and the strains were further characterized using some advanced techniques (phase contrast microscopy, Raman spectroscopy, Fourier-transform infrared spectroscopy, and scanning electron microscope). For maximum biofilm formation by isolated bacterial strains, many physiochemical factors including incubation duration, temperature, pH, carbon source concentration, and nitrogen source concentration were further examined and optimized. The fact that these non-pathogenic strains were found in public water supplies is another important part of this research because there is a chance that they could change into pathogenic state in future and cause disease in humans.