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Factors Affecting Pregnancy Success in the Bitch Following Transcervical Insemination.

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Fresh semen yielded the highest pregnancy rates and largest litter sizes in endoscopic-assisted transcervical inseminations (TCIs). This study evaluated TCIs using fresh, chilled, and frozen-thawed semen to guide canine breeding recommendations.

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Area of Science:

  • Veterinary Reproduction
  • Canine Artificial Insemination
  • Reproductive Technologies

Background:

  • Endoscopic-assisted transcervical insemination (TCI) is a widely adopted technique in canine reproduction.
  • Evaluating the efficacy of different semen types is crucial for optimizing breeding outcomes.

Purpose of the Study:

  • To retrospectively analyze data from endoscopic-assisted transcervical inseminations (TCIs).
  • To compare pregnancy rates and litter sizes across fresh, chilled, and frozen-thawed semen.
  • To provide data-driven recommendations for canine breeding protocols.

Main Methods:

  • Retrospective analysis of 137 fresh, 67 chilled, and 63 frozen-thawed semen TCI cases (2018-2021).
  • Semen evaluation included sperm count, total motility, and progressive motility.
  • Pregnancy confirmed by ultrasonography; litter size determined by radiography.

Main Results:

  • Pregnancy rates were highest with fresh semen (83.21%), followed by chilled (67.16%) and frozen-thawed (66.67%).
  • Litter size was significantly larger with fresh semen (6.82 puppies) compared to chilled (5.21) and frozen-thawed (4.59).
  • No significant difference in pregnancy rates was observed based on clinician or sedation use.

Conclusions:

  • Fresh semen provides superior pregnancy rates and litter sizes in TCI procedures.
  • Endoscopic-assisted transcervical insemination protocols can be optimized by considering semen type.
  • Findings support informed decision-making for canine breeders aiming to maximize reproductive success.