Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic Analysis of Organotypic Porcine Retina Cultures.

International journal of molecular sciences·2026
Same author

The Mia40 substrate Mix17 exposes its N-terminus to the cytosolic side of the mitochondrial outer membrane.

Journal of cell science·2025
Same author

The UbiB family member Cqd1 forms a novel membrane contact site in mitochondria.

Journal of cell science·2023
Same author

The MICOS complex, a structural element of mitochondria with versatile functions.

Biological chemistry·2020
Same author

Msp1 cooperates with the proteasome for extraction of arrested mitochondrial import intermediates.

Molecular biology of the cell·2020
Same author

HOPS recognizes each SNARE, assembling ternary <i>trans</i>-complexes for rapid fusion upon engagement with the 4th SNARE.

eLife·2020

Related Experiment Video

Updated: Jul 24, 2025

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.4K

An Improved Method to Isolate Mitochondrial Contact Sites.

Siavash Khosravi1, Johanna Frickel2, Max E Harner3

  • 1Department of Cell Biology, Biomedical Center, Medical Faculty, Ludwig-Maximilians University Munich; Cardio-Metabolic Diseases, Boehringer Ingelheim Pharma GmbH & Co. KG.

Journal of Visualized Experiments : Jove
|July 3, 2023
PubMed
Summary
This summary is machine-generated.

Researchers isolated mitochondrial contact sites using yeast mitochondria to identify key proteins. This method revealed the MICOS complex and a novel contact site, advancing our understanding of mitochondrial communication and function.

More Related Videos

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.1K
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
10:12

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Published on: November 13, 2015

12.9K

Related Experiment Videos

Last Updated: Jul 24, 2025

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.4K
Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.1K
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
10:12

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Published on: November 13, 2015

12.9K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Mitochondrial Biology

Background:

  • Mitochondria are vital eukaryotic organelles with diverse functions beyond energy production, including apoptosis and biosynthesis.
  • Mitochondrial dysfunction is linked to severe human diseases like cancer, diabetes, and neurodegeneration.
  • Mitochondrial membranes must interact via protein contact sites to facilitate cellular communication.

Purpose of the Study:

  • To develop and refine a method for isolating mitochondrial contact sites.
  • To identify novel protein candidates involved in mitochondrial membrane interactions.
  • To elucidate the composition and function of key mitochondrial contact sites.

Main Methods:

  • Utilized Saccharomyces cerevisiae mitochondria for the isolation of contact sites.
  • Employed a technique to identify proteins localized at these contact sites.
  • Applied an improved method to discover new contact site components.

Main Results:

  • Successfully identified the mitochondrial contact site and cristae organizing system (MICOS) complex.
  • The MICOS complex, crucial for inner mitochondrial membrane organization, is conserved across species.
  • A novel contact site involving Cqd1 and the Por1-Om14 complex was discovered.

Conclusions:

  • The developed method is effective for identifying mitochondrial contact site proteins.
  • The discovery of the MICOS complex and the novel Cqd1-Por1-Om14 complex deepens the understanding of mitochondrial structure and function.
  • These findings provide new targets for investigating mitochondrial diseases.