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Related Experiment Video

Updated: Jul 24, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Modelling Human Post-Implantation Development via Extra-Embryonic Niche Engineering.

Joshua Hislop^1,2,3, Amir Alavi^4,5, Qi Song^4,5

¹Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Biorxiv : the Preprint Server for Biology

|July 3, 2023

Summary

This summary is machine-generated.

Researchers developed iDiscoids, a novel human stem cell model, to study early post-implantation development. This breakthrough allows for better understanding of embryonic and extra-embryonic tissue formation and disease modeling.

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Area of Science:

Developmental Biology
Stem Cell Biology
Human Embryogenesis

Background:

Human embryo implantation is crucial for development but difficult to study in vivo.
Existing stem cell models lack comprehensive embryonic and extra-embryonic tissue development.
Understanding early post-implantation is key for developmental toxicology and disease modeling.

Approach:

Developed iDiscoids from human induced pluripotent stem cells using a synthetic gene circuit.
Created a model for reciprocal co-development of embryonic and engineered extra-embryonic tissues.
Utilized a high-throughput, scalable, and reproducible platform.

Key Points:

iDiscoids self-organize and form tissue boundaries, recapitulating key developmental events.
Observed yolk sac-like tissue specification with extra-embryonic mesoderm and hematopoietic characteristics.
Demonstrated bilaminar disc-like embryonic morphology, amniotic cavity formation, and axis patterning.

Conclusions:

iDiscoids provide a tractable human model for studying early post-implantation development.
This platform facilitates research into drug testing, developmental toxicology, and disease modeling.
Enables mechanistic insights into a critical window of human life previously inaccessible.