Green Approach for the Simultaneous Synthesis and Separation of Gold Nanoparticles

Gold nanoparticles (AuNPs) have diagnostic and therapeutic applications as they are biocompatible and can be surface-functionalized. The use of organic solvents in the synthesis of AuNPs hampers their applications in the medicinal field. The large-scale production of nanoparticles requires their simultaneous synthesis and separation. Self-assembly of nanoparticles at the fluid-fluid interface facilitates their separation from the bulk and eliminates a downstream processing step. In this work, we exploit this in an aqueous two-phase system (ATPS) to synthesize and separate stable AuNPs. The ATPS was based on polyethylene glycol (PEG) and trisodium citrate dihydrate (citrate) as both these compounds can reduce Au ions. After the synthesis of nanoparticles, using one of the solutes, a complementary solution containing the other solute is added to form a two-phase system to facilitate self-assembly at the interface. The nanoparticles synthesized in different phases are characterized using UV-visible spectroscopy, scanning electron microscopy, and transmission electron microscopy. The AuNPs synthesized using the citrate solution are found to be unstable. Particles synthesized using the ATPS with PEG-600 are trapped at the interface while those using PEG-6000 remain in the bulk. Continuous synthesis and separation of nanoparticles in slug flow in a millichannel are demonstrated as a first step for large-scale controlled synthesis.