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Acacetin restrains the malignancy of esophageal squamous carcinoma cells via regulating JAK2/STAT3 pathway

Wei Wang1,2, Zhang Renquan1

  • 1Department of Thoracic Surgery, First Affiliated Hospital, Anhui Medical University, Hefei, An Hui, China.

Chemical Biology & Drug Design
|July 4, 2023
PubMed

Insights

Acacetin, a plant flavonoid, effectively inhibits esophageal squamous cell carcinoma growth and spread. It promotes apoptosis and blocks the JAK2/STAT3 signaling pathway, offering a potential therapeutic strategy.

Area of Science:

  • Natural product chemistry
  • Oncology
  • Molecular biology

Background:

  • Acacetin is a flavonoid with known anti-inflammatory and anti-cancer properties.
  • Esophageal squamous cell carcinoma (ESCC) is an aggressive cancer with limited treatment options.

Purpose of the Study:

  • To investigate the anti-cancer effects of acacetin on esophageal squamous carcinoma cells.
  • To elucidate the molecular mechanisms underlying acacetin's action in ESCC.

Main Methods:

  • In vitro experiments evaluated proliferation, migration, invasion, and apoptosis of ESCC cell lines (TE-1, TE-10) treated with acacetin.
  • Bioinformatics analysis predicted genes involved in acacetin's effects on ESCC.
  • Western blot assessed apoptosis-related proteins (Bax, Bcl-2) and JAK2/STAT3 pathway proteins.

Main Results:

  • Acacetin significantly inhibited the proliferation, migration, and invasion of TE-1 and TE-10 cells.
  • Acacetin treatment promoted apoptosis by increasing Bax expression and decreasing Bcl-2 expression.
  • Acacetin was found to inhibit the JAK2/STAT3 signaling pathway in ESCC cells.

Conclusions:

  • Acacetin demonstrates potent anti-cancer activity against esophageal squamous carcinoma cells in vitro.
  • The anti-cancer effects of acacetin are mediated, in part, by the induction of apoptosis and the inhibition of the JAK2/STAT3 pathway.
  • Acacetin represents a promising therapeutic agent for esophageal squamous cell carcinoma, warranting further investigation.
Keywords:
AcacetinJAK2/STAT3 signaling pathwayapoptosisesophageal carcinomamigrationproliferation

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