Related Experiment Video
Updated: Jul 24, 2025
12:20
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
11.9K
Multiple gene detection using a selective fluorophore probe-RNA hybridization/graphene oxide quenching system
Tasnima Alam Asa1, Young Jun Seo1
1Department of Chemistry, Jeonbuk National University, Jeonju 54896, South Korea. yseo@jbnu.ac.kr.
The Analyst
|July 5, 2023
Summary
This study presents a rapid, sensitive assay for detecting multiple RNA virus genes using ligation-double transcription and fluorometric profiling. The novel method enables precise diagnosis of RNA-virus diseases with high accuracy in just 45 minutes.
Area of Science:
- Molecular Biology
- Biotechnology
- Diagnostic Assays
Background:
- Accurate and rapid detection of RNA viruses is crucial for disease diagnosis and control.
- Current methods may lack the sensitivity or specificity required for complex viral diagnostics.
- Identifying multi-gene classifiers can improve diagnostic precision for RNA-virus-related diseases.
Purpose of the Study:
- To develop a novel assay for multiple-gene recognition and detection.
- To demonstrate the utility of this assay for identifying multi-gene classifiers for diagnostic purposes.
- To expedite the precise diagnosis of RNA-virus-related diseases.
Main Methods:
- Ligation-double transcription mediated fluorometric profiling.
- Selective fluorophore probe-RNA hybridization coupled with graphene oxide quenching.
- Detection of distinct viral genes for broad RNA virus identification.
Main Results:
- The assay achieved high sensitivity (369.6–408 copies/mL for SARS-CoV-2 genes).
- Demonstrated high specificity, distinguishing targets with up to two mismatches.
- Completed total experimentation within 45 minutes.
- Successfully detected various RNA viruses in diverse sample pools.
Conclusions:
- The developed assay is efficient, sensitive, and specific for multiple-gene recognition.
- This method can identify multi-gene classifiers for improved RNA virus diagnostics.
- The system has the potential to significantly advance the diagnosis of RNA-virus-related diseases.
Related Concept Videos
02:34DNA Microarrays
17.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.6K
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
02:07FISH - Fluorescent In-situ Hybridization
20.8K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
20.8K