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Electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose composite gels and their visualization by
Analytical Biochemistry
|July 1, 1986
Abstract:
We have developed techniques for the electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose gel slabs and subsequent fixation, impregnation, and fluorography of such electrophoretograms. The procedure permits the examination of newly synthesized proteoglycan subspecies using a rapid technique, previously unavailable for these labeled molecules.