Related Experiment Video
Updated: Jul 24, 2025
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
Hsa_circ_0000129 knockdown attenuates proliferation and migration in keloid fibroblasts by targeting miR-485-3p/SGMS2
Zi Li1, Wenhui Zhang2, Heting Zhang3
1Department of Orthopedics & Plastic Surgery, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan 430015, Hubei, China.
Background:
Aberrant biofunction of circular RNAs (circRNAs) is potently implicated in keloid formation. However, their roles have been underinvestigated. Recent evidence has demonstrated the pro-tumor role of circ_0000129 in cancers, and yet its role in keloid remains elusive.
Methods:
RT-qPCR analysis and or western blotting of miR-485-3p, circ_0000129, and SGMS2 in keloid tissues and keloid fibroblasts was implemented. CCK8, EdU, scratch wound healing, and Transwell migration assays were perfomed to determine the keloid fibroblast proliferation and migration. Luciferase reporter and RIP assays were adopted to analyze the interaction among circ_0000129, miR-485-3p and SGMS2.
Results:
In keloid tissues and keloid fibroblasts, circ_0000129 and SGMS2 were amplified, although miR-485-3p expression was downregulated. Furthermore, siRNAs-targeting endogenous circ_0000129 resulted in proliferation and migration defect of keloid fibroblasts. MiR-485-3p was simultaneously recognized by circ_0000129 and SGMS2 3'UTR. Rescued functional assays also illustrated that miR-485-3p loss was beneficial to the proliferation and migration of keloid fibroblasts, and these promoting changes were nullified by accompanied silence circ_0000129 or SGMS2.
Conclusion:
Circ_0000129 sponges miR-485-3p and releases expression of SGMS2 from the miR-485-3p suppression, promoting migration and proliferation of keloid fibroblasts, suggesting targeting circ_0000129/miR-485-3p/SGMS2 might be a promising strategy against keloid fibroblasts.
Availability Of Data And Material:
All data generated or analyzed during this study are included in this article.
Insights
Circular RNA circ_0000129 promotes keloid fibroblast proliferation and migration by sponging miR-485-3p and upregulating SGMS2. Targeting this pathway may offer a novel therapeutic strategy for keloid treatment.
Area of Science:
- Molecular Biology
- Dermatology
- Biochemistry
Background:
- Aberrant circular RNA (circRNA) function is linked to keloid formation, but specific roles remain understudied.
- circ_0000129, known to promote cancer, has an unelucidated role in keloid pathogenesis.
Purpose of the Study:
- To investigate the role and mechanism of circ_0000129 in keloid fibroblast proliferation and migration.
- To elucidate the interaction between circ_0000129, miR-485-3p, and SGMS2 in keloid formation.
Main Methods:
- Quantitative real-time PCR and Western blotting were used to assess gene and protein expression.
- Cellular assays including CCK8, EdU, scratch wound healing, and Transwell migration were performed.
- Luciferase reporter and RNA immunoprecipitation assays were employed to determine molecular interactions.
Main Results:
- circ_0000129 and SGMS2 were upregulated, while miR-485-3p was downregulated in keloid tissues and fibroblasts.
- Silencing circ_0000129 inhibited keloid fibroblast proliferation and migration.
- circ_0000129 directly sponged miR-485-3p, which in turn suppressed SGMS2 expression.
Conclusions:
- circ_0000129 acts as a molecular sponge for miR-485-3p, relieving SGMS2 suppression and promoting keloid fibroblast proliferation and migration.
- The circ_0000129/miR-485-3p/SGMS2 axis presents a potential therapeutic target for keloid treatment.
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