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Macrophage migration inhibitory factor-mediated mast cell extracellular traps induce inflammatory responses upon
Hiroyuki Tada1, Takashi Nishioka2, Rina Ishiyama1
1Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.
Abstract:
Mast cell extracellular traps (MCETs) released by mast cells contribute to host defense. In this study, we investigated the effects of MCETs released from mast cells after infection with a periodontal pathogen Fusobacterium nucleatum. We found that F. nucleatum induced MCET release from mast cells, and that MCETs expressed macrophage migration inhibitory factor (MIF). Notably, MIF bound to MCETs induced proinflammatory cytokine production by monocytic cells. These findings suggest that MIF expressed on MCETs, released from mast cells upon infection with F. nucleatum, promotes inflammatory responses that may be associated with the pathogenesis of periodontal disease.
Insights
Mast cell extracellular traps (MCETs) released during Fusobacterium nucleatum infection express macrophage migration inhibitory factor (MIF). This MIF on MCETs promotes inflammatory responses, potentially contributing to periodontal disease pathogenesis.
Area of Science:
- Immunology
- Microbiology
- Periodontology
Background:
- Mast cells play a role in host defense through the release of mast cell extracellular traps (MCETs).
- Periodontal disease is often associated with bacterial infections, including those caused by Fusobacterium nucleatum.
Purpose of the Study:
- To investigate the role of MCETs released by mast cells in response to Fusobacterium nucleatum infection.
- To determine the expression of macrophage migration inhibitory factor (MIF) on MCETs and its functional consequences.
Main Methods:
- Mast cells were infected with Fusobacterium nucleatum to induce MCET release.
- MCETs were analyzed for the expression of macrophage migration inhibitory factor (MIF).
- The effect of MIF-bound MCETs on monocytic cells and proinflammatory cytokine production was assessed.
Main Results:
- Fusobacterium nucleatum infection induced the release of MCETs from mast cells.
- MCETs were found to express macrophage migration inhibitory factor (MIF).
- MIF bound to MCETs stimulated proinflammatory cytokine production in monocytic cells.
Conclusions:
- MCETs released upon Fusobacterium nucleatum infection express MIF.
- MIF on MCETs contributes to the inflammatory response in periodontal infections.
- These findings suggest a mechanism linking MCETs, MIF, and the pathogenesis of periodontal disease.
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