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Expression of NEDD4L and ENaC in Urinary Extracellular Vesicles in Pre-eclampsia
P Y M Leung1,2,3, M Katerelos2,3, S Choy2
1Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Australia.
Objective:
To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls.
Methods:
Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, α-ENaC and γ-ENaC were identified by immunoblotting.
Results:
There was no difference in the expression of NEDD4L (p = 0.17) and α-ENaC (p = 0.10). PE subjects showed increased expression of γ-ENaC by 6.9-fold compared to NP (p < 0.0001).
Conclusion:
ENaC expression is upregulated in UEV of pre-eclamptic subjects but was not associated with changes in NEDD4L.
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