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Multi-parameter surface plasmon resonance instrument for multiple nucleic acid quantitative detection
Huixiang Wang1, Honggang Wang1, Yafeng Huang1
1College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Biomedical Microdevices
|July 7, 2023
Summary
This study introduces a rapid, label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection. The novel system enables quick and accurate identification of biomarkers like miRNA-21 and miRNA-141, crucial for cancer diagnostics.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Biosensing Technology
Background:
- Multiplex nucleic acid assays are vital for disease diagnosis, environmental monitoring, and food safety.
- Traditional methods face limitations including complex operations, long detection times, unstable labeling, and signal interference.
- There is a need for rapid, sensitive, and user-friendly multiplex nucleic acid detection platforms.
Purpose of the Study:
- To develop a real-time, rapid, label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection.
- To address the challenges of multiplex detection and quantitative analysis in complex biological samples.
- To provide a simple and efficient point-of-care testing (POCT) platform for small molecules like DNA and miRNA.
Main Methods:
- Development of a multiparametric optical system using total internal reflection, a linear light source, prism, photodetector, and mechanical transmission.
- Implementation of an adaptive threshold consistency correction algorithm to manage channel variations and enable quantitative comparisons.
- Utilizing surface plasmon resonance (SPR) for label-free and amplification-free detection of target nucleic acids.
Main Results:
- The SPR instrument successfully achieved label-free and amplification-free detection of miRNA-21 and miRNA-141 in 30 minutes.
- The system demonstrated good repeatability and specificity, with a limit of detection (LOD) of 50 nM for target oligonucleotides.
- The smallest detectable absolute amount of sample was approximately 4 pmol, highlighting high sensitivity.
Conclusions:
- The developed SPR instrument offers a simple, efficient, and rapid solution for multiplex nucleic acid detection.
- This technology has the potential to serve as a point-of-care testing (POCT) platform for various small molecules, including cancer biomarkers.
- The label-free and amplification-free approach overcomes key limitations of traditional nucleic acid assays.
