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Related Experiment Video

Updated: Jun 17, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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A Low-Cost Handheld Centrifugal Microfluidic System for Multiplexed Visual Detection Based on Isothermal

Nan Wang1, Xiaobin Dong1, Yijie Zhou1

  • 1Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Sensors (Basel, Switzerland)
|August 10, 2024
PubMed
Summary

A new low-cost, handheld device enables multiplexed visual detection using recombinase polymerase amplification (RPA). This system offers rapid, on-site testing for multiple targets, including African swine fever virus (ASFV).

Keywords:
RPAcentrifugal microfluidichandheldmultiplexedvisual detection

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Point-of-care diagnostics require portable and cost-effective solutions.
  • Multiplexed detection enhances diagnostic efficiency by analyzing multiple targets simultaneously.
  • Recombinase polymerase amplification (RPA) offers rapid, isothermal nucleic acid amplification suitable for field applications.

Purpose of the Study:

  • To develop a low-cost, handheld centrifugal microfluidic system for multiplexed visual detection.
  • To integrate RPA amplification with visual detection in a portable device.
  • To demonstrate the system's capability for detecting multiple targets, such as African swine fever virus (ASFV) genes.

Main Methods:

  • Development of a compact centrifugal microfluidic chip with four parallel reaction units.
  • Integration of heating, optical, rotation, and sensing modules into a handheld companion device.
  • Utilized non-contact heating, magnetic sensing-controlled motor for mixing and centrifugation, and optimized visual fluorescence detection.

Main Results:

  • The system successfully performed multiplexed RPA amplification and visual detection.
  • Achieved a limit of detection (LOD) of 75 copies/test for ASFV genes, comparable to tube-based RPA.
  • Demonstrated direct visual observation of results using eyes or a smartphone.

Conclusions:

  • The developed handheld system provides a cost-effective and portable solution for multiplexed visual detection.
  • This technology has potential for rapid, on-site diagnostics in resource-limited settings.
  • The system enables efficient detection of multiple nucleic acid targets, including specific viral genes.