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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 24, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

A Method for Rapid, Quantitative Evaluation of Particle Sorting in Microfluidics Using Basic Cytometry Equipment.

Robert Salomon^1,2, Sajad Razavi Bazaz^1,2,3, Wenyan Li²

¹Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

|July 8, 2023

Summary

This summary is machine-generated.

This study presents a flow cytometry method to accurately measure microfluidic particle separation. This technique quantifies performance for single cells and clusters, even in complex mixtures.

Keywords:

cell sorting cytometry microfluidics separation

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Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Current methods for assessing microfluidic separation devices have limitations in accuracy and applicability to complex samples.
High-speed imaging and manual cell counting are insufficient for precise performance characterization, especially with high concentrations or cell clusters.

Purpose of the Study:

To introduce and detail a novel method using flow cytometry for quantitative performance evaluation of continuous-flow microfluidic particle separation devices.
To overcome limitations of existing methods by enabling accurate assessment in complex, high-concentration mixtures.

Main Methods:

Utilizing flow cytometry with pulse processing to quantitatively analyze particle separation.
Applying the method to assess both single cells and cell clusters, such as circulating tumor cell (CTC) clusters.
Integrating cell surface phenotyping for detailed analysis of complex cell mixtures.

Main Results:

The developed flow cytometry method accurately quantifies separation efficiencies and sample purities.
It successfully assesses device performance for both individual cells and cell clusters in complex mixtures.
The method demonstrates superior capability compared to traditional techniques like hemocytometers or cell counters.

Conclusions:

This flow cytometry-based approach provides a robust and accurate method for evaluating microfluidic separation devices.
It enables precise characterization of devices intended for separating single cells and biologically relevant clusters.
The method will accelerate the development and validation of advanced microfluidic separation technologies.