Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Lactobacillus plantarum</i> ameliorates high-fat-diet-induced dyslipidemia <i>via</i> cholesterol metabolism and gut microbiota modulation.

Food & function·2026
Same author

AI-Guided Design and Predictive Modeling of Synthetic <i>Escherichia coli</i> Promoters through Comprehensive -10/-35 Box Engineering.

ACS synthetic biology·2026
Same author

Deep learning guided programmable design of Escherichia coli core promoters from sequence architecture to strength control.

Nucleic acids research·2025
Same author

Developing PhoR-PhoB-Based Biosensor by Directed Evolution for Application in Ultralow Inorganic Phosphorus Detection.

ACS sensors·2025
Same author

Engineering Xylose Metabolism and Coutilization with Glucose in <i>Corynebacterium glutamicum</i> for Efficient Microbial Cell Factories.

Journal of agricultural and food chemistry·2025
Same author

Metabolic Engineering of <i>Escherichia coli</i> for the Improved Malonic Acid Production.

ACS synthetic biology·2025
Same journal

Perfusion development and its potential for cell therapy manufacturing with adherent cells.

Applied microbiology and biotechnology·2026
Same journal

Bacterial degradation of aromatic ester pollutants in agro-ecosystems: implications for bioremediation.

Applied microbiology and biotechnology·2026
Same journal

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026
Same journal

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials.

Applied microbiology and biotechnology·2026
Same journal

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains.

Applied microbiology and biotechnology·2026
Same journal

Ammonium and nitrate fluxes drive metabolic functional shifts in tap water biofilms.

Applied microbiology and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jul 24, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K

Biosensor-based high-throughput screening enabled efficient adipic acid production.

Rui Zhi1,2, Nan Cheng1,2, Guohui Li3,4

  • 1National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Applied Microbiology and Biotechnology
|July 8, 2023
PubMed
Summary
This summary is machine-generated.

We developed a biosensor and screening platform to improve bio-based adipic acid production, overcoming genetic limitations. This led to a 3.62 g/L titer in a 5-L fermenter, advancing sustainable chemical manufacturing.

Keywords:
Adipic acidBiosensorGenetic heterogeneityHigh-throughput screeningReverse adipate degradation pathway

More Related Videos

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

Related Experiment Videos

Last Updated: Jul 24, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Synthetic Biology

Background:

  • Industrial adipic acid synthesis poses environmental pollution risks.
  • Bio-based production offers a sustainable alternative but faces challenges from genetic heterogeneity impacting product yield.
  • Improving strain performance is crucial for the industrialization of bio-based chemicals.

Purpose of the Study:

  • To overcome genetic heterogeneity limiting bio-based adipic acid production.
  • To develop a high-throughput screening platform for identifying high-performance strains.
  • To enhance adipic acid titers through metabolic engineering and fermentation optimization.

Main Methods:

  • Constitutive expression of the reverse adipate degradation pathway.
  • Design and optimization of an adipic acid biosensor.
  • Establishment of a high-throughput screening platform utilizing the biosensor.
  • Fermentation optimization and scale-up studies.

Main Results:

  • Screened a high-performance strain achieving an initial adipic acid titer of 188.08 mg·L⁻¹.
  • Optimized fermentation led to a titer of 531.88 mg·L⁻¹ in shake flasks (18.78-fold improvement).
  • Scale-up fermentation in a 5-L fermenter yielded 3.62 g·L⁻¹ of adipic acid.

Conclusions:

  • The developed strategies effectively reduce genetic heterogeneity in microbial strains.
  • The high-throughput screening platform is a valuable tool for industrial strain development.
  • This work provides a foundation for more efficient industrial bio-production of adipic acid.