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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

483
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Updated: Sep 9, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Developing PhoR-PhoB-Based Biosensor by Directed Evolution for Application in Ultralow Inorganic Phosphorus

Wenyan Cao1,2, Xuan Zhou1,2, Chao Huang1,2

  • 1School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.

ACS Sensors
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a sensitive biosensor using the PhoR-PhoB system for detecting inorganic phosphorus (Pi) in water and serum. Optimized biosensor variants show a significantly enhanced dynamic range for precise ultralow Pi detection.

Keywords:
PhoR-PhoB systembiosensordirected evolutionenvironmental monitoringinorganic phosphorusserum Pi detection

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Inorganic phosphorus (Pi) is essential for life but elevated levels in water and serum cause environmental and health issues.
  • Existing biosensors for Pi detection have limitations in dynamic range and detection range, hindering practical application.
  • The Escherichia coli PhoR-PhoB two-component system naturally senses Pi.

Purpose of the Study:

  • To develop a highly sensitive biosensor for ultralow inorganic phosphorus (Pi) detection.
  • To overcome the limitations of the native PhoR-PhoB system's dynamic and detection ranges.
  • To enable precise Pi measurement in environmental and clinical samples.

Main Methods:

  • Optimization of PhoR and PhoB expression levels to enhance the biosensor's dynamic range.
  • Directed evolution of the PhoR kinase domain to create a mutant with improved sensitivity.
  • Application of the optimized biosensor for Pi detection in serum and environmental water samples.

Main Results:

  • Optimized expression increased the biosensor's dynamic range by 97-fold.
  • Directed evolution yielded a PhoR mutant (L222F/N307S/Q344H) with a 232-fold dynamic range.
  • The engineered biosensor achieved a linear detection range of 0.012-0.07 mM, suitable for serum and wastewater standards.

Conclusions:

  • The engineered PhoR-PhoB biosensor offers enhanced sensitivity and a wider dynamic range for Pi detection.
  • The developed biosensor is effective for precise ultralow Pi measurement in both serum and environmental water.
  • This technology holds potential for monitoring and managing Pi levels in diverse settings.