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Updated: Jul 24, 2025
Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion
Evelyn Lattmann1, Valérie Lapaire1, Mitchell P Levesque1
1Department of Dermatology, University of Zurich, University Hospital Zurich, Schlieren, ZH 8952, Switzerland.
Abstract:
Characterization of extracellular vesicles (EVs) holds great promise for biomarker discovery and understanding of diseases, including melanoma, the deadliest skin cancer type. Here, we describe a size-exclusion chromatography method to isolate and concentrate EVs from patient material including (1) patient-derived melanoma cell line supernatants and (2) plasma and serum biopsies. Additionally, we provide a protocol to analyze EVs by nano-flow cytometry. EV suspensions obtained with the presented protocol can be used for several downstream analyses including RNA sequencing and proteomics.
