Compound probiotics producing cellulase could replace cellulase preparations during solid-state fermentation of...

Area of Science:

• Agricultural Science
• Biotechnology
• Food Science

Background:

• Agricultural by-products are often underutilized.
• Fermentation and enzymatic hydrolysis can convert low-value by-products into high-value products.
• Enzyme costs limit the industrial application of enzymatic hydrolysis.

Purpose of the Study:

• To evaluate the efficacy of compound probiotics producing cellulase (CPPC) compared to cellulase preparations for millet bran fermentation.
• To assess the impact of CPPC on fiber structure, metabolite content, and anti-nutrient factors in millet bran.
• To provide a theoretical basis for the efficient utilization of agricultural by-products.

Main Methods:

• Solid-state fermentation of millet bran using cellulase preparation.
• Solid-state fermentation of millet bran using CPPC.
• Analysis of crude fiber content, beneficial metabolites, anti-nutrient factors, and microbial populations.
• Correlation analysis of microbial growth.

Main Results:

• Both cellulase preparation and CPPC reduced crude fiber content by 23.78% and 28.32%, respectively.
• Both treatments increased beneficial metabolites and microorganisms.
• CPPC demonstrated superior performance in reducing anti-nutrient factors and increasing anti-inflammatory metabolites.
• Synergistic growth was observed between Lactiplantibacillus and Issatchenkia during fermentation.

Conclusions:

• CPPC can effectively replace cellulase preparations in millet bran fermentation.
• CPPC enhances antioxidant properties and reduces anti-nutrient factors in millet bran.
• This study provides a theoretical reference for the valorization of agricultural by-products using microbial fermentation.