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Effect Of Elastomeric Separator On Microbial Count In Gingival Crevicular Fluid
Samia Sattar Khan1, Saad Asad2, Umar Hussain3
1Freelancer.
Journal of Ayub Medical College, Abbottabad :, JAMC
|July 9, 2023
Summary
Orthodontic separators increase bacteria in gingival crevicular fluid (GCF). Chlorhexidine mouthwash and saline irrigation significantly reduced bacterial counts, with chlorhexidine being more effective.
Area of Science:
- Orthodontics
- Periodontology
- Microbiology
Background:
- Orthodontic separators precede band placement, posing a risk of bacteremia.
- Bacterial proliferation in gingival crevicular fluid (GCF) is a concern during separator use.
Purpose of the Study:
- To evaluate the impact of separators on GCF bacterial counts.
- To assess the efficacy of chlorhexidine mouthwash and saline irrigation in mitigating bacterial increase.
Main Methods:
- A randomized controlled trial involving 51 healthy participants (18-25 years) with good oral hygiene.
- Three groups: brushing only (control), saline irrigation, and 2% chlorhexidine mouthwash.
- GCF bacterial counts were measured at baseline, day 3, and day 7 post-separator placement.
Main Results:
- Bacterial counts in GCF significantly decreased in both saline and chlorhexidine groups by day 3 and day 7 (p<0.001).
- Both interventions showed significant reduction compared to the control group.
- Chlorhexidine demonstrated the greatest reduction in bacterial count, outperforming saline.
Conclusions:
- Separator placement leads to an increase in GCF bacterial load.
- Chlorhexidine mouthwash is more effective than saline irrigation in reducing bacterial counts post-separator placement.

