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Development of a cellulose-based 96-well plate vertical flow pull-down assay
Hoi Lok Cheng1, Huan Jia1, Sing Mei Lim1
1Antimicrobial Resistance Interdisciplinary Research Group (AMR-IRG), Singapore-MIT Alliance in Research and Technology (SMART), 1 CREATE Way, 138602, Singapore.
Analytical Methods : Advancing Methods and Applications
|July 10, 2023
Summary
Researchers developed a high-throughput cellulose paper assay for point-of-care (PoC) diagnostics. This 96-well plate assay enables simultaneous testing of multiple samples, overcoming limitations of single-sample PoC devices.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Diagnostic Technology
Background:
- Cellulose paper is an abundant and low-cost biomaterial suitable for point-of-care (PoC) diagnostic tests.
- Current cellulose-based PoC tests are limited by low sample processing throughput, restricting their application scope.
Purpose of the Study:
- To develop a high-throughput cellulose-based assay for PoC diagnostics.
- To overcome the single-sample limitation of existing PoC tests and expand their applicability.
Main Methods:
- Development of a 96-well plate vertical flow pull-down assay using patterned cellulose paper.
- The assay design features pre-patterned cellulose paper requiring no pre-immobilization of capturing reagents.
- Incorporation of a reusable housing for the cellulose paper device.
Main Results:
- Successful development of a high-throughput cellulose-based 96-well plate assay.
- The assay can process 96 tests simultaneously, significantly increasing throughput.
- The assay is easy to prepare and customizable for various detection targets.
Conclusions:
- The developed cellulose-based 96-well plate assay offers a high-throughput solution for PoC diagnostics.
- This assay can be applied to laboratory testing, population surveillance, and large clinical trials.
- The technology enhances the applicability of cellulose-based PoC tests for broader diagnostic needs.
