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Updated: May 8, 2026

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
A Surgical Technique for Anterior Cruciate Ligament Reconstruction Using Semitendinosus Graft: An All-Inside
Toshiaki Takahashi1, Seiji Watanabe2, Toshio Ito3
1Department of Sports and Health Science, Faculty of Collaborating Regional Innovation, Ehime University, Matsuyama, Ehime, Japan.
Abstract:
Given the paucity of reports on all-inside reconstruction procedures via the transfemoral approach, we describe a minimally invasive, all-inside transfemoral technique that enables creating femoral and tibial sockets from the intra-articular cavity. Our transfemoral approach makes it possible to sequentially create femoral and tibial sockets using the same reamer bit, while a single drilling guide is set in place. Our custom socket drilling guide was designed to integrate with a tibial tunnel guide, which helped locate the tunnel exit at an anatomically acceptable location. The advantages of this method include easy and precise positioning of the femoral tunnel, narrow tibial tunnel, minimal damage to the intramedullary trabecular bone integrity, and low postoperative risks of pain, bleeding, and infections.

