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Protocol for calcium imaging of dorsal and ventral CA1 neurons in head-fixed mice
Miru Yun1, Jonghan Shin1, Min Whan Jung1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea; Center for Synaptic Brain Dysfunctions, Institute for Basic Science, Daejeon 34141, Korea.
Abstract:
In contrast to other techniques utilized in physiological studies, calcium imaging can visualize target neurons located deep in the brain. Here, we present a protocol for one-photon calcium imaging of dorsal and ventral CA1 neurons in head-fixed mice. We describe procedures for injecting GCaMP6f virus, implanting a gradient-index (GRIN) lens, and installing a baseplate for Inscopix microscope mounting. For complete details on the use and execution of this protocol, please refer to Yun et al.1.
