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Updated: Jul 24, 2025

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Published on: October 31, 2012
Establishment of induced pluripotent stem cell line SDPHi003-A from a healthy male donor
Yue Xi1, Haiyan Zhang2, Xiaomeng Yang2
1First School of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China; Tumor Minimally Invasive Comprehensive Treatment Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Ji'nan 250021, China; Pediatric Research Institute, Children's Hospital Affiliated to Shandong University, Ji'nan 250022, China.
Abstract:
Peripheral blood mononuclear cells (PBMCs) were derived from a 67-year-old Chinese male. We used non-integrating episomal vectors containing OCT4, SOX2, KLF4 and c-MYC to reprogram PBMCs into induced pluripotent stem cell (iPSCs). This iPSC line, SDPHi003-A, have a normal karyotype, expressed pluripotent markers and have the potential for trilineage differentiation. This iPSC line can be used as a control for disease modeling studies and contribute to the research exploring disease pathogenesis.
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