Activity-based protein profiling in microbes and the gut microbiome.
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Current Opinion in Chemical Biology
|July 10, 2023
Summary
Activity-based protein profiling (ABPP) uses chemical probes to study protein function in biological systems. Recent advances in ABPP are enhancing the discovery of new antibiotics and understanding microbial communities.
Area of Science:
- Biochemistry
- Chemical Biology
- Microbiology
Background:
- Activity-based protein profiling (ABPP) is a chemical method to investigate protein function and enzyme activity.
- ABPP employs activity-based probes that covalently bind to target proteins, enabling their identification.
- Mass spectrometry-based proteomics, utilizing click chemistry or affinity-based labeling, is key for analyzing tagged proteins.
Purpose of the Study:
- To review recent advancements in ABPP.
- To highlight applications of ABPP in bacterial research.
- To discuss the utility of ABPP in studying complex microbial communities.
Main Methods:
- Design and utilization of activity-based probes with reactive warheads.
- Application of mass spectrometry-based proteomic platforms.
- Enrichment of tagged proteins via click chemistry or affinity-based labeling.
Main Results:
- ABPP has been instrumental in understanding bacterial biological processes.
- This chemical approach has aided in the discovery of novel antibiotics.
- ABPP facilitates the characterization of host-microbe interactions.
Conclusions:
- ABPP is a versatile chemical strategy for probing protein function and enzymatic activity.
- Recent ABPP developments offer significant potential for bacterial research and antibiotic discovery.
- The review emphasizes the growing importance of ABPP in analyzing microbial communities.
More Related Videos
08:09An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
8.8K
09:02Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
Published on: April 20, 2018
19.9K
Related Concept Videos
01:18Amino Acid Catabolism
56
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
56
01:33Proteomics
7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K