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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Related Experiment Video

Updated: Jul 24, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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CRISPR enables heritable genome editing in planta.

Tofazzal Islam1, Shamfin Hossain Kasfy1

  • 1Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh.

Trends in Genetics : TIG
|July 10, 2023
PubMed
Summary

New clustered regularly interspaced short palindromic repeats (CRISPR) genome editing techniques enable rapid, transgene-free crop improvements in a single generation. This technology bypasses traditional breeding, accelerating the development of enhanced commercial crops.

Keywords:
crop improvementgraftingmobile CRISPR constructspeed breedingviral vectors

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Area of Science:

  • Agricultural Science
  • Genetics
  • Biotechnology

Background:

  • Traditional crop improvement relies on lengthy breeding processes and tissue culture.
  • Genome editing offers a potential alternative for faster crop development.

Purpose of the Study:

  • To highlight recent advancements in CRISPR-based genome editing for plants.
  • To emphasize the efficiency and speed of these new editing methods.

Main Methods:

  • Utilizing clustered regularly interspaced short palindromic repeats (CRISPR) technology.
  • Focusing on in planta genome editing techniques.

Main Results:

  • Demonstrated rapid and efficient genome editing in plants.
  • Achieved heritable, transgene-free edits within one generation.

Conclusions:

  • CRISPR-based genome editing significantly accelerates crop improvement.
  • These methods present a valuable tool for enhancing commercially important crops efficiently.