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Related Experiment Video

Updated: Jul 24, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Profiling Single-Cell Genome and Transcriptome by scONE-Seq.

Lei Yu¹, Angela Ruohao Wu^2,3

¹Division of Life Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.

Methods in Molecular Biology (Clifton, N.J.)

|July 10, 2023

Summary

This summary is machine-generated.

We developed scONE-seq, a versatile method for simultaneous single-cell transcriptome and genome profiling. This technique is compatible with frozen tissues, enabling deeper insights into tumor heterogeneity for research and drug discovery.

Keywords:

Frozen tissue Single-cell DNA-seq Single-cell RNA-seq Single-cell multi-omics Tumor heterogeneity

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Last Updated: Jul 24, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

Biotechnology
Genomics
Cancer Research

Background:

Tumor cellular heterogeneity presents challenges in understanding cancer.
Single-cell multi-omics technologies offer novel insights into cellular diversity.
Access to patient samples, particularly from biobanks, is crucial for research.

Purpose of the Study:

To introduce scONE-seq, a novel method for simultaneous transcriptome and genome profiling at the single-cell level.
To demonstrate the versatility and compatibility of scONE-seq with frozen tissue samples from biobanks.
To provide detailed procedures for implementing scONE-seq for research applications.

Main Methods:

Developed a one-tube reaction method for simultaneous profiling of single-cell/nucleus transcriptome and genome.
Ensured compatibility of the scONE-seq method with frozen tissue samples.
Validated sequencing library compatibility with both Illumina and MGI platforms.

Main Results:

scONE-seq enables simultaneous transcriptome and genome profiling from single cells or nuclei.
The method is compatible with frozen tissue, expanding its applicability to biobank samples.
Generated sequencing libraries suitable for widely used sequencing platforms.

Conclusions:

scONE-seq is a versatile tool for studying tumor heterogeneity.
The method's compatibility with frozen tissues enhances its utility for large-scale research and drug discovery.
scONE-seq facilitates comprehensive single-cell multi-omics analysis.