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Effect of arachidonic acid on pre- and post-hatching in vitro bovine embryo development
L González-Brusi1, A Pérez-Gómez1, A C Quiroga1
1Animal Reproduction Department, INIA, CSIC, Madrid 28040, Spain.
Context:
Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development.
Aims:
To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro -produced bovine embryos.
Methods:
Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100μM AA up to Day 12.
Key Results:
Pre-hatching development to blastocyst was completely abrogated at 333μM AA, whereas blastocyst rates and cell numbers were not altered at 100μM AA. Impaired post-hatching development was observed at 100μM AA, whereas no effect on survival rates was noted at 5, 10 and 20μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5-10μM AA. AA exposure downregulated the genes PTGIS , PPARG , LDHA and SCD in Day 12 embryos.
Conclusions:
Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development.
Implications:
AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages.
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