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Updated: Jul 23, 2025
04:46
Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
Published on: February 3, 2023
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Acteoside improves adipocyte browning by CDK6-mediated mTORC1-TFEB pathway
Yunxia Sun1, Xintao Ni2, Siyao Cheng2
1School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China; Academy of Chinese Medical Science, Zhejiang Chinese Medical University, China.
Summary
Acteoside (Act) promotes adipocyte browning, a process that increases energy expenditure. This natural compound activates a pathway involving CDK6, mTOR, and TFEB, leading to enhanced thermogenesis and potential obesity treatment.
Area of Science:
- Metabolic research
- Cell biology
- Pharmacology
Background:
- Adipocyte browning enhances energy expenditure via thermogenesis, offering a strategy against obesity and metabolic disorders.
- Phytochemicals that promote adipocyte thermogenesis are gaining attention for their therapeutic potential.
- Acteoside (Act), a phenylethanoid glycoside from plants, is known to modulate metabolic functions.
Purpose of the Study:
- To investigate the adipocyte browning effects of Acteoside (Act).
- To elucidate the molecular mechanisms underlying Act-induced adipocyte browning.
Main Methods:
- Stimulating beige adipocyte differentiation from stromal vascular fraction (SVF) of inguinal white adipose tissue (iWAT) and 3T3-L1 preadipocytes.
- Assessing the conversion of mature white adipocytes to beige adipocytes.
- Analyzing the involvement of the CDK6-mTOR-TFEB pathway and downstream targets like PGC-1α and UCP1.
Main Results:
- Act promotes adipocyte browning through both differentiation of progenitor cells and direct conversion of mature white adipocytes.
- Act inhibits CDK6 and mTOR, leading to reduced phosphorylation and increased nuclear retention of TFEB.
- This mechanism results in the induction of PGC-1α and subsequent UCP1-dependent browning.
Conclusions:
- Acteoside effectively induces adipocyte browning via a novel CDK6-mTORC1-TFEB signaling pathway.
- This pathway regulates TFEB nuclear translocation, promoting mitochondrial biogenesis and thermogenesis.
- Act represents a promising phytochemical for developing strategies against obesity and metabolic diseases.
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