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Updated: Jul 23, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
Determination of copolymer compositions in polyhydroxyalkanoates using 1H benchtop nuclear magnetic resonance
Juan F Araneda1, Hasitha de Alwis Weerasekera2, Matthew C Leclerc1
1Nanalysis Corp., 1-4600 5 St NE, Calgary, AB T2E 7C3, Canada. matt.leclerc@nanalysis.com.
Abstract:
Continuous research into polyhydroxyalkanoates (PHAs), biodegradable polymers which can be produced by, and harvested from, various bacteria has led to more cost-effective ways of isolating and commercializing them. As bio-based polymers, PHAs can be transformed into compostable bioplastics and utilized for a variety of applications. Often isolated as copolymers, the monomeric ratio compositions of these products greatly affect both the properties and consequently, possible end uses of these products. As such, methods of reliably characterizing these ratios are important for quality control and product development purposes. Herein, we discuss how 1H benchtop nuclear magnetic resonance (NMR) instruments can be used for the determination of monomeric ratio compositions in PHAs and compare results obtained at three different NMR field strengths: 1.40 T (60 MHz), 2.35 T (100 MHz), and 9.4 T (400 MHz).
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