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Published on: May 18, 2017
Effect of Pre-Slaughter Transport Stress on Protein S-Nitrosylation Levels of Pork during Postmortem Aging
Chao Ma1, Wangang Zhang1, Lei Zhou1
1Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
This study aimed to investigate the effect of pre-slaughter transport stress on the protein S-nitrosylation levels of pork during 0, 3, and 6 days of aging. Pigs (N = 16) were randomly selected and divided into two treatments: 3 h transport (transport stress, TS) and 3 h transport followed by 3 h resting (control, CON). Results showed that the TS group at 0 and 3 days presented a higher nitric oxide synthase (NOS) activity and neuronal NOS (nNOS) expression than the CON group (P < 0.05). Additionally, nNOS was not only abundantly localized in the membrane but also observed in small amounts in the cytoplasm. The immunoblot of overall S-nitrosylated protein showed that protein S-nitrosylation levels in the TS group were greater than in the CON group during postmortem aging (P < 0.05). This work can deliver novel insights into the mechanism of meat quality changes in response to pre-slaughter stress.
