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Flow-force relationships in mitochondrial oxidative phosphorylation.

H Woelders, J Putters, K van Dam

    FEBS Letters
    |August 11, 1986
    PubMed
    Summary
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    Mitochondrial oxidation and phosphorylation rates depend on protonmotive force (ΔμH+). Unique relationships exist, but the probe triphenylmethylphosphonium (TPMP+) showed anomalous behavior under certain conditions.

    Area of Science:

    • Biochemistry
    • Mitochondrial Physiology
    • Membrane Biophysics

    Background:

    • Mitochondria generate ATP through oxidative phosphorylation, a process driven by the protonmotive force (ΔμH+).
    • Understanding the precise relationship between ΔμH+ and mitochondrial function is crucial for cellular energy metabolism research.

    Purpose of the Study:

    • To investigate the quantitative relationships between protonmotive force (ΔμH+) and the rates of mitochondrial oxidation and phosphorylation.
    • To assess the reliability of different probes for measuring membrane potential (Δψ) under varying conditions.

    Main Methods:

    • Isolated rat-liver mitochondria were used to measure oxidation and phosphorylation rates.
    • Protonmotive force (ΔμH+) was varied, and its components, membrane potential (Δψ) and pH gradient, were assessed.

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  • Membrane potential (Δψ) was determined using potassium ion (K+) distribution with valinomycin and the probe triphenylmethylphosphonium (TPMP+).

    • Main Results:

    • The rates of oxidation and phosphorylation showed a steep, consistent dependence on ΔμH+ regardless of how ΔμH+ was altered.
    • When Δψ was calculated from K+ distribution, flow-force relationships were consistent.
    • Using TPMP+ as a probe for Δψ yielded slightly different flow-force relationships, indicating potential probe-specific anomalies.

    Conclusions:

    • Unique and consistent relationships exist between the protonmotive force (ΔμH+) and mitochondrial oxidation/phosphorylation rates.
    • The behavior of the triphenylmethylphosphonium (TPMP+) probe for membrane potential (Δψ) can be anomalous under certain experimental conditions, affecting derived flow-force relationships.