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Binding of cardiotoxin analogue III from Formosan cobra venom to FL cells
The binding equilibrium at 37 or 0 degrees C of 125I-cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37 degrees C was irreversible. The Scatchard analysis at 37 degrees C on the binding of 125I-CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high-affinity sites was 1.1 X 10(10) mol-1 or 2.8 X 10(6) per FL cell, respectively. At 37 or 0 degrees C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37 degrees C was inhibited by the presence of acidic phospholipids.
The binding equilibrium at 37 or 0 degrees C of 125I-cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37 degrees C was irreversible. The Scatchard analysis at 37 degrees C on the binding of 125I-CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high-affinity sites was 1.1 X 10(10) mol-1 or 2.8 X 10(6) per FL cell, respectively. At 37 or 0 degrees C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37 degrees C was inhibited by the presence of acidic phospholipids.