Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hypoxia rescues complex 1-associated disease caused by proteostatic defects.

Nature metabolism·2026

Same author

RNA G-quadruplexes function as a tunable switch of FUS phase separation.

Nucleic acids research·2026

Same author

Isoform-specific steric zippers drive aberrant assembly and mislocalization of shortened TDP-43.

Science advances·2026

Same author

Darwin's solution: Honeybees survive mite vectors and viruses through natural selection.

Trends in parasitology·2026

Same author

The local mechanostructural properties of protein cargoes regulate nucleocytoplasmic transport.

Nature physics·2026

Same author

Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration.

Science (New York, N.Y.)·2026

Search research articles

Related Experiment Video

Updated: Jul 23, 2025

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Transcriptome-wide RNA binding analysis of C9orf72 poly(PR) dipeptides.

Rubika Balendra^1,2, Igor Ruiz de Los Mozos^3,4,5, Hana M Odeh⁶

¹UK Dementia Research Institute at UCL, London, UK.

Life Science Alliance

|July 12, 2023

Summary

This summary is machine-generated.

Poly(PR) protein, linked to neurodegenerative diseases like ALS and FTD, binds specific RNA sequences. This binding, particularly to GAAGA motifs, may drive disease pathology.

More Related Videos

Measuring RAN Peptide Toxicity in C. elegans

Measuring RAN Peptide Toxicity in C. elegans

Published on: April 30, 2020

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

Related Experiment Videos

Last Updated: Jul 23, 2025

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Measuring RAN Peptide Toxicity in C. elegans

Measuring RAN Peptide Toxicity in C. elegans

Published on: April 30, 2020

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

Area of Science:

Neuroscience
Genetics
Molecular Biology

Background:

An intronic GGGGCC repeat expansion in *C9orf72* is a leading genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
Sense and antisense transcription of these repeats produce dipeptide repeat proteins (DPRs), including poly(GA), poly(GR), and poly(PR), implicated in neurodegeneration.
Poly(PR) interaction with RNA is hypothesized to contribute to its toxicity, but its transcriptome-wide binding profile remains uncharacterized.

Purpose of the Study:

To identify the RNA targets of poly(PR) on a transcriptome-wide scale using crosslinking and immunoprecipitation (CLIP) analysis.
To investigate the binding affinity and potential functional consequences of poly(PR)-RNA interactions, particularly concerning specific RNA motifs.

Main Methods:

Crosslinking and immunoprecipitation (CLIP) in human cells to map poly(PR) RNA binding sites.
Bioinformatic analysis to identify enriched sequences at binding sites.
In vitro binding assays and phase separation experiments to assess poly(PR) interaction with specific RNA sequences.

Main Results:

Poly(PR) was found to bind to nearly 600 distinct RNA molecules in human cells.
The sequence motif GAAGA was significantly enriched at poly(PR) binding sites.
In vitro studies demonstrated that poly(GAAGA) RNA exhibits higher binding affinity for poly(PR) and promotes its phase separation into condensates.

Conclusions:

Poly(PR) exhibits preferential binding to RNAs containing GAAGA motifs.
This specific RNA interaction may play a crucial role in the pathogenesis of C9orf72-associated neurodegenerative diseases.
Further research into these poly(PR)-RNA interactions could reveal novel therapeutic targets.