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Updated: Jul 23, 2025

07:45
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Published on: June 6, 2022
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In situ decrypting plasmonic nanoparticle size-controlled phosphorylation of epidermal growth factor receptor in
Hongyan Wang1, Yan Ding1, Yu Zhang2
1First Affiliated Hospital of Anhui Medical University, Hefei 230000, China.
Abstract:
Recently, interaction between epidermal growth factor receptor (EGFR) and EGFR-targeted nanoprobes is a hot topic. Here, we use dark field microscope (DFM) observe different aggregations of EGFR-targeted nanoprobes in diverticulum. Different aggregation states are related to phosphorylation of EGFR. EGFR phosphorylation can be adjusted by gold nanoparticles (GNPs) size.

