Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

638
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
638
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

594
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
594
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

753
Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
753
Electrodes: Overview01:17

Electrodes: Overview

1.7K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
1.7K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

268
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
268
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

998
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
998

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-nucleus transcriptomics identifies SPON1 as a candidate mediator of the anti-fibrotic effect of <i>Abelmoschus manihot</i> (L.) in diabetic kidney disease.

American journal of translational research·2026
Same author

Innovative peptide-loaded hybrid membrane nanovaccine enables precise personalized immunotherapy for HPV-related cervical diseases.

Signal transduction and targeted therapy·2026
Same author

Advances in extraction, purification, and pharmacological mechanisms of calycosin: a comprehensive review.

Chinese journal of natural medicines·2026
Same author

Dietary oxidative balance and renal impairment in diabetes identified by machine learning and functional analysis.

Frontiers in nutrition·2026
Same author

Diallyl trisulfide activates Nrf2 by promoting S-sulfhydration of Keap1 to attenuate acute hypobaric hypoxia-induced lung injury.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Relationships Between Leaf Coloration Changes, Cellular Structure, Photosynthetic Physiology, and Hydraulic Traits in <i>Liquidambar formosana</i> Hance Under Drought Stress in Autumn.

Plants (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jul 23, 2025

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

14.1K

Self-Calibrated Ion-Selective Electrodes.

Nafesa Adil1, Renjie Wang1, Xuewei Wang1

  • 1Department of Chemistry, Virginia Commonwealth University, 1001 W. Main Street, Richmond, Virginia 23284, United States.

Analytical Chemistry
|July 13, 2023
PubMed
Summary
This summary is machine-generated.

A new ion-selective electrode (ISE) simplifies decentralized sensing with a built-in calibration component, eliminating fluid handling. This self-calibrating sensor offers reliable ion measurements by using relative electromotive force (EMF) changes.

More Related Videos

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.3K
Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito
11:30

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito

Published on: August 24, 2021

2.9K

Related Experiment Videos

Last Updated: Jul 23, 2025

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

14.1K
Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
08:03

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

17.3K
Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito
11:30

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito

Published on: August 24, 2021

2.9K

Area of Science:

  • Electrochemistry
  • Sensor Technology
  • Analytical Chemistry

Background:

  • Ion-selective electrode (ISE) potentiometry requires on-site calibration, hindering decentralized applications.
  • Complex calibration procedures limit the widespread use of ISEs in point-of-care or field settings.

Purpose of the Study:

  • To develop a novel ISE with a fluidics-free, built-in self-calibration mechanism.
  • To enable reliable and simplified ion measurements without external standard solutions.

Main Methods:

  • Designed an ISE with integrated indicator and reference electrodes connected by an ionic conductor.
  • Established a baseline electromotive force (EMF) for one-point calibration.
  • Utilized sample-induced EMF change relative to the baseline for ion quantification.

Main Results:

  • Achieved low relative EMF variability (<2.0 mV) in potentiometric sensors.
  • Demonstrated that the self-calibration bridge does not compromise Nernstian response.
  • Validated accuracy in undiluted human blood samples against a commercial blood gas analyzer.

Conclusions:

  • The developed self-calibrating ISE offers a simplified and reliable approach for decentralized ion sensing.
  • The fluidics-free calibration strategy is adaptable to various sensor configurations.
  • This innovation addresses key limitations of conventional ISEs for broader implementation.