QIAGEN's Investigator^® 24plex QS and GO! PCR Amplification

The Investigator^® 24Plex kits are multiplex PCR kits utilized by forensic laboratories to simultaneously amplify 22 of the most commonly utilized STR markers for human identity testing, including the 20 core CODIS loci, along with the sex marker Amelogenin and 2 novel quality sensors. These quality sensors are unique internal PCR controls that provide useful insight to the analyst regarding possible inhibition or degradation within the sample. This chapter describes the use of the QS version of the kit designed for use with extracted DNA from casework samples, as well as the use of the GO! version of the kit designed for direct amplification of reference samples.