QIAGEN's Investigator^® Quantiplex^® Pro Kit

The QIAGEN Investigator^® Quantiplex^® Pro Kit is a real-time quantitative PCR assay utilized by forensic DNA laboratories to determine the amount of amplifiable human and male DNA in a sample prior to downstream amplification of specific STR markers for human identity testing. This quantification method includes two internal controls that assist the analyst in a preliminary evaluation of the sample in regard to both inhibition or degradation that may be present in the sample and subsequently affect the more targeted downstream amplification of specific markers for identity testing. The internal controls are analogous to the quality sensors contained in QIAGEN's Investigator^® 24plex line of amplification kits, ensuring that the sample's performance in the quantitation step can accurately predict the success of the STR amplification results. This chapter describes the physical plate setup of a quantitative PCR assay utilizing the QIAGEN Investigator^® Quantiplex^® Pro Kit as well as the steps and software configurations involved in running such a plate on the Applied Biosystems 7500 Real-Time PCR System for Human Identification using HID Real-Time PCR Analysis Software v1.1 or 1.2.