Population dynamics and molecular adaption of Tetranychus cinnabarinus to long-term thermal stress

Global warming is a general trend in the current era. Temperature is one of the most important nonbiological factors that affects the development, life cycle and distribution of arthropods, which are a major component of agriculture pests. This study focused on life-table parameters and the molecular adaption of Tetranychus cinnabarinus under long-term thermal stress.

The life tables of T. cinnabarinus were constructed at room temperature (26 °C) and high temperature (34 °C). Results showed that although the lifespan of the mites was shortened, the developmental periods of egg, larva and nymph stages were accelerated, and the peak egg-laying period came earlier at high temperature, which resulted in faster expansion of pest mite population. RNA-seq was used to reveal the thermal adaption mechanism according to differentially expressed genes. Combined with transcriptome data and quantitative polymerase chain reaction (qPCR) verification, MAPK, CAT, HSP20 and HSP70 were found highly expressed at 34 °C, which were associated with thermal adaption of T. cinnabarinus. RNAi analysis proved that expression of HSP20 was closely related to the survival of mites at high temperature.

These results indicated that long-term high temperature treatment was beneficial to the expansion of the T. cinnabarinus population. The genes involved in heat tolerance of T. cinnabarinus such as MAPK-HSP pathway provides ideas for subsequent control measures. © 2023 Society of Chemical Industry.