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Updated: Aug 6, 2026
Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling
Published on: April 16, 2015
Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric patients undergoing
Yaling Liu1,2, Huigang Shu2, Ping Wan3
1Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road Suzhou, Jiangsu, China.
Insights
High levels of NET components in serum indicate a higher risk of pulmonary complications and death in children after liver transplants. These NET components can predict these adverse outcomes.
Area of Science:
- Immunology
- Transplantation Medicine
- Critical Care Medicine
Background:
- Parental liver transplantation (PLT) is vital for pediatric hepatic failure, but postoperative pulmonary complications (PPCs) remain a concern.
- The underlying mechanisms driving PPCs after PLT are not fully understood.
- Investigating biomarkers for PPCs is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the association between neutrophil extracellular trap (NET) components and PPCs in pediatric patients undergoing PLT.
- To evaluate NET components as potential biomarkers for predicting PPCs, prolonged ICU stay, and one-year mortality.
- To elucidate the role of NETs in the pathophysiology of PPCs post-PLT.
Main Methods:
- A cohort of 133 pediatric patients undergoing PLT was studied.
- Serum levels of NET components, including circulating free DNA (cfDNA), DNA-histone complex, and myeloperoxidase (MPO)-DNA complex, were measured.
- The incidence of PPCs, duration of ICU stay, and one-year mortality were recorded as outcomes.
Main Results:
- The overall incidence of PPCs was 47.4%.
- Elevated serum levels of cfDNA, DNA-histone complexes, and MPO-DNA complexes were significantly associated with increased risk of PPCs, prolonged ICU stay, and one-year mortality.
- NET components demonstrated strong predictive value for PPCs, with AUCs ranging from 0.813 to 0.906.
Conclusions:
- High serum levels of NET components are linked to increased PPCs and one-year mortality in pediatric PLT recipients.
- NET components can serve as valuable biomarkers for predicting post-PLT PPCs.
- These findings highlight NETs as potential therapeutic targets and prognostic indicators in pediatric liver transplantation.
Background:
Parental liver transplantation (PLT) improves long-term survival rates in paediatric hepatic failure patients; however, the mechanism of PLT-induced postoperative pulmonary complications (PPCs) is unclear.
Methods:
A total of 133 paediatric patients undergoing PLT were included. Serum levels of NET components, including circulating free DNA (cfDNA), DNA-histone complex, and myeloperoxidase (MPO)-DNA complex, were detected. The occurrence of PPCs post-PLT, prolonged intensive care unit (ICU) stay and death within one year were recorded as the primary and secondary outcomes.
Results:
The overall rate of PPCs in the hospital was 47.4%. High levels of serum cfDNA, DNA-histone complexes and MPO-DNA complexes were associated with an increased risk of PPCs (for cfDNA, OR 2.24; for DNA-histone complex, OR 1.64; and for MPO-DNA, OR 1.94), prolonged ICU stay (OR 1.98, 4.26 and 3.69, respectively), and death within one year (OR 1.53, 2.65 and 1.85, respectively). The area under the curve of NET components for the prediction of PPCs was 0.843 for cfDNA, 0.813 for DNA-histone complexes, and 0.906 for MPO-DNA complexes. During the one-year follow-up, the death rate was higher in patients with PPCs than in patients without PPCs (14.3% vs. 2.9%, P = 0.001).
Conclusions:
High serum levels of NET components are associated with an increased incidence of PPCs and death within one year in paediatric patients undergoing PLT. Serum levels of NET components serve as a biomarker for post-PLT PPCs and a prognostic indicator.
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