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Updated: Jul 23, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
Conversion of a Single-Frequency X-Band EPR Spectrometer into a Broadband Multi-Frequency 0.1-18 GHz Instrument for
1Department of Biotechnology, Delft University of Technology, Building 58, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Abstract:
A broadband EPR spectrometer is an instrument that can be tuned to many microwave frequencies over several octaves. Its purpose is the collection of multi-frequency data, whose global analysis affords interpretation of complex spectra by means of deconvolution of frequency-dependent and frequency-independent interaction terms. Such spectra are commonly encountered, for example, from transition-metal complexes and metalloproteins. In a series of previous papers, I have described the development of broadband EPR spectrometers around a vector network analyzer. The present study reports on my endeavor to start from an existing X-band spectrometer and to reversibly re-build it into a broadband machine, in a quest to drastically reduce design effort, building costs, and operational complexity, thus bringing broadband EPR within easy reach of a wide range of researchers.
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