Fast, Multiple-Use Optical Biosensor for Point-of-Care Glucose Detection with Mobile Devices Based on Bienzyme...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensor Technology

Background:

• Non-invasive glucose monitoring is crucial for diabetes management.
• Current methods often require invasive procedures or complex equipment.
• Point-of-care testing needs affordable, sensitive, and rapid diagnostic tools.

Purpose of the Study:

• To develop and analyze a novel colorimetric biosensor for glucose detection in human urine.
• To evaluate the analytical performance and stability of the developed biosensor.
• To explore the potential for non-invasive, personal diabetes screening.

Main Methods:

• Synthesis of porous polyamide microparticles as a support matrix.
• Immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) enzymes.
• Development of a colorimetric assay using 3,3',5,5'-tetramethylbenzidine (TMB) chromogen and CIELab image processing.

Main Results:

• The consecutively immobilized enzyme system (GH@PA-C) demonstrated superior activity.
• A linear correlation was observed between color difference and glucose concentration (0.01–3.0 mM) in urine.
• The biosensor exhibited rapid response (20 s), a low detection limit (30.7 µM), wide pH stability (4–8), and long-term storage stability (30 days).
• The biosensor allowed for multiple consecutive uses without significant performance degradation.

Conclusions:

• The developed colorimetric biosensor is effective for non-invasive glucose determination in urine.
• Its high sensitivity, stability, reusability, and ease of use support its application in point-of-care diagnostics.
• This technology holds potential for routine, personal diabetes monitoring and early detection.