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Updated: Jul 23, 2025
08:43
Metagenomic Analysis of Silage
Published on: January 13, 2017
18.4K
Clinical metagenomics-challenges and future prospects.
Maliha Batool1, Jessica Galloway-Peña1
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States.
Frontiers in Microbiology
|July 14, 2023
Summary
Metagenomic next-generation sequencing (mNGS) offers a sensitive approach for diagnosing rare infections. However, challenges in sensitivity, cost, and standardization must be addressed for widespread clinical adoption.
Area of Science:
- Infectious Diseases
- Genomics
- Clinical Diagnostics
Background:
- Undiagnosed infections pose significant risks, leading to mortality and long-term health consequences.
- Current diagnostic methods often fail to identify rare, novel, or complex causative pathogens.
- A universal diagnostic strategy is crucial to reduce the burden of undocumented infections.
Purpose of the Study:
- To review the efficacy and limitations of metagenomic next-generation sequencing (mNGS) in diagnosing severe infections.
- To compare mNGS with conventional diagnostic methods.
- To propose strategies for optimizing mNGS as a clinical diagnostic tool.
Main Methods:
- Culture-independent analysis using metagenomic next-generation sequencing (mNGS).
- Review of existing studies and case reports on mNGS in clinical diagnosis.
- Examination of mNGS performance metrics including sensitivity, specificity, and cost-effectiveness.
Main Results:
- mNGS demonstrates high sensitivity in detecting rare, novel, and unexpected pathogens without prior assumptions.
- Studies confirm mNGS's effectiveness in improving clinical diagnosis for various infections.
- Limitations include challenges in sensitivity, specificity, cost, bioinformatic pipeline standardization, and result interpretation.
Conclusions:
- mNGS is a powerful tool for identifying the causes of undiagnosed infections.
- Addressing current limitations is essential for integrating mNGS into routine clinical practice.
- Further development is needed to maximize mNGS's potential in diagnosing severe infections.
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