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Published on: March 15, 2021
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Applications of CRISPR technology in cellular immunotherapy.
Xiaoyu Zhou1,2,3, Paul A Renauer1,2,3, Liqun Zhou1,2,3,4
1Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, USA.
Immunological Reviews
|July 14, 2023
Summary
CRISPR gene editing revolutionizes immunology and cancer research by enabling direct manipulation of immune cells. This technology accelerates the discovery of new drug targets and drives advancements in cellular immunotherapies with numerous clinical trials underway.
Area of Science:
- Immunology
- Genetics
- Biotechnology
Background:
- CRISPR technology offers unprecedented capabilities for precise genomic manipulation.
- Its application in immunology and cancer research is rapidly expanding.
- Understanding immune cell function is crucial for developing novel therapies.
Purpose of the Study:
- To review the development and applications of CRISPR gene editing in immune cells.
- To highlight its role in functional genomics screening and cellular immunotherapy.
- To discuss future directions in this evolving field.
Main Methods:
- Review of CRISPR-based gene editing and screening methodologies.
- Analysis of CRISPR applications in immune cell research.
- Examination of CRISPR-driven cellular immunotherapy strategies.
Main Results:
- CRISPR enables unbiased functional genetic screens in immune cells.
- It facilitates the discovery of novel regulators of immune responses.
- CRISPR-engineered immune cells are central to numerous clinical trials.
Conclusions:
- CRISPR technology has transformed immune cell research and therapy development.
- Functional genomics screening with CRISPR aids in identifying new therapeutic targets.
- CRISPR-based cellular immunotherapies show significant promise and are advancing rapidly.
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