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Related Experiment Video

Updated: Jul 23, 2025

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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The Protocol of Circulating Tumor Cell Detection.

Bingbing Zhao1, Ling Zhang1, Zhequan Liu1

  • 1Genesis, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|July 14, 2023
PubMed
Summary

Detecting circulating tumor cells (CTCs) aids in tumor development assessment and offers applications in early diagnosis and prognosis prediction. This article details CTC detection methods, including negative enrichment and immunofluorescence in situ hybridization.

Keywords:
Circulating tumor cellFISHNegative enrichment

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Area of Science:

  • Oncology
  • Biomedical Engineering
  • Molecular Diagnostics

Background:

  • Circulating tumor cells (CTCs) are crucial indicators of tumor development and metastasis.
  • CTCs play a significant role in various clinical applications, including early cancer diagnosis, postoperative monitoring, treatment evaluation, and prognosis prediction.

Purpose of the Study:

  • To provide a comprehensive overview of the entire process of circulating tumor cell (CTC) detection.
  • To highlight the key methodologies involved in isolating and detecting CTCs.

Main Methods:

  • Focuses on negative enrichment strategies for isolating CTCs from biological samples.
  • Details the application of immunofluorescence in situ hybridization (FISH) for the detection and characterization of CTCs.

Main Results:

  • The study outlines a complete workflow for CTC detection, from sample preparation to final analysis.
  • Emphasizes the importance of both enrichment and detection techniques for accurate CTC identification.

Conclusions:

  • CTC detection is a valuable tool in oncology, supporting clinical decision-making across the patient care continuum.
  • The described methods, negative enrichment and immunofluorescence in situ hybridization, are integral to effective CTC analysis.