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Updated: Jul 23, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
Published on: January 26, 2024
Predicting Protein Interaction Sites Using PITHIA
SeyedMohsen Hosseini1, Lucian Ilie2
1Department of Computer Science, University of Western Ontario, London, ON, Canada.
Abstract:
Several proteins work independently, but the majority work together to maintain the functions of the cell. Thus, it is crucial to know the interaction sites that facilitate protein-protein interactions. The development of effective computational methods is essential because experimental methods are expensive and time-consuming. This chapter is a guide to predicting protein interaction sites using the program "PITHIA." First, some installation guides are presented, followed by descriptions of input file formats. Afterward, PITHIA's commands and options are outlined with examples. Moreover, some notes are provided on how to extend PITHIA's installation and usage.
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