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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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A 96-well plate UV fluorometer based on micro fluorescence detector array and dynamic zero correction algorithm.

Chuanliang Wang1, Xuhui Geng2, Yan Gao2

  • 1Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Talanta
|July 14, 2023
PubMed
Summary

A novel 96-well plate UV fluorometer utilizes an 8-channel detector array for rapid analysis. Its dynamic zero correction algorithm significantly enhances accuracy and detection limits for high-throughput screening.

Keywords:
96-Well plate UV fluorometerDynamic zero correction algorithmMicro fluorescence detector array

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • Accurate and efficient fluorescence detection is crucial for high-throughput screening in various scientific fields.
  • Existing fluorometers face challenges with background fluorescence and measurement accuracy.

Purpose of the Study:

  • To develop and evaluate a novel 96-well plate UV fluorometer for enhanced detection efficiency and accuracy.
  • To address limitations in measurement accuracy caused by background fluorescence variations.

Main Methods:

  • Development of an 8-channel detector array using UV light emitting diodes (LEDs) and photodiodes (PDs).
  • Implementation of ray tracing for optical path design to prevent well crosstalk.
  • Introduction of a dynamic zero correction algorithm to mitigate background fluorescence effects.
  • Simultaneous scanning and detection across 8 channels for time efficiency.

Main Results:

  • Achieved a scanning time of approximately 80 seconds for a 96-well plate.
  • Demonstrated a measurement repeatability (RSD) of 2.25% for a 1 μg/L standard.
  • Significantly improved limit of detection (LOD), repeatability, and reduced average relative error compared to fixed zero correction.

Conclusions:

  • The developed UV fluorometer offers robust performance and potential for high-throughput analysis.
  • The dynamic zero correction algorithm enhances measurement accuracy and reliability.
  • This instrument shows promise for applications in food safety and life sciences requiring rapid detection.