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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Related Experiment Video

Updated: Jul 23, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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Public archiving of volume EM data.

Andrii Iudin1, Matthew Hartley1, Gerard J Kleywegt1

  • 1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridgeshire, United Kingdom.

Methods in Cell Biology
|July 14, 2023
PubMed
Summary
This summary is machine-generated.

Archiving valuable volume electron microscopy (vEM) datasets offers significant benefits for researchers and the scientific community. EMBL-EBI provides services to support data archival and future data utilization.

Keywords:
BioImage archiveCorrelative microscopyData archivingEMPIARElectron microscopyFAIR standardsMetadata standardsMultimodal imagingStandardizationVolume EMVolume electron microscopyvEM

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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Related Experiment Videos

Last Updated: Jul 23, 2025

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Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
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Area of Science:

  • Cellular and Molecular Biology
  • Microscopy Techniques
  • Data Science

Background:

  • Volume electron microscopy (vEM) generates scientifically valuable datasets.
  • Generating these datasets is time-consuming and resource-intensive.
  • Public archival of vEM data is crucial for scientific progress.

Purpose of the Study:

  • To discuss the benefits of public archival for vEM datasets.
  • To explain EMBL-EBI's image data services for vEM and correlative imaging data archival.
  • To explore future developments for unlocking value from vEM datasets.

Main Methods:

  • Literature review on vEM dataset archival benefits.
  • Description of EMBL-EBI's image data archival services.
  • Discussion of potential future advancements in vEM data utilization.

Main Results:

  • Public archival enhances data accessibility and reusability.
  • EMBL-EBI offers robust services for vEM and correlative imaging data.
  • Future developments promise increased value extraction from vEM datasets.

Conclusions:

  • Archiving vEM datasets is essential for the scientific community.
  • EMBL-EBI plays a key role in facilitating data archival.
  • Continued development will maximize the impact of vEM research.