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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Updated: Jul 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Enhancing CRISPR/Cas systems with nanotechnology.

Rupali Chowdhry1, Steven Z Lu2, Seungheon Lee3

  • 1Department of Public Health, The University of Texas at Austin, Austin, TX 78712, USA.

Trends in Biotechnology
|July 14, 2023
PubMed
Summary
This summary is machine-generated.

Nanotechnology enhances CRISPR/Cas systems for improved cellular entry, stability, and targeted delivery in diagnostics and therapeutics. This integration promises more sensitive detection and personalized treatments, overcoming current limitations.

Keywords:
CRISPRdiagnosticsgene editingnanotechnologytherapeutics

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Area of Science:

  • Biotechnology and Nanomedicine
  • Molecular Biology and Gene Editing

Background:

  • CRISPR/Cas systems offer revolutionary potential in biology and medicine, enabling new disease diagnostics and therapeutics.
  • Current CRISPR/Cas systems face limitations including poor cellular entry, instability in biological settings, and off-target effects.

Purpose of the Study:

  • To explore the integration of nanotechnology with CRISPR/Cas systems to overcome existing limitations.
  • To highlight advancements in using nanomaterials to improve CRISPR/Cas system performance for diagnostics and therapeutics.

Main Methods:

  • Review of recent scientific literature on CRISPR/Cas systems and nanotechnology integration.
  • Analysis of how nanomaterials address challenges like cellular delivery, stability, and specificity.
  • Examination of nanotech-enhanced CRISPR/Cas for diagnostic platforms and therapeutic applications.

Main Results:

  • Nanotechnology integration enhances CRISPR/Cas systems for preferential tissue accumulation and tunable properties.
  • Nanomaterials improve CRISPR/Cas stability and cellular entry, reducing off-target effects.
  • Nanomaterials enable faster, more sensitive, and convenient CRISPR/Cas-mediated detection platforms.

Conclusions:

  • Integrating nanotechnology with CRISPR/Cas systems is a promising strategy to overcome current limitations.
  • Further development is needed to fully realize the potential of nanotech-enhanced CRISPR/Cas technologies.
  • This interdisciplinary approach holds significant promise for advancing diagnostics and therapeutics.