Unexpectedly high nitrate levels in a pristine forest river on the Southeastern Qinghai-Tibet Plateau

River nitrate (NO₃^-) pollution is a global environmental issue. Recently, high NO₃^- levels in some pristine or minimally-disturbed rivers were reported, but their drivers remain unclear. This study integrated river isotopes (δ¹⁸O/δ¹⁵N-NO₃^- and δD/¹⁸O-H₂O), ¹⁵N pairing experiments, and qPCR to reveal the processes driving the high NO₃^- levels in a nearly pristine forest river on the Qinghai-Tibet Plateau. The river isotopes suggested that, at the catchment scale, NO₃^- removal was prevalent in summer, but weak in winter. The pristine forest soils contributed more than 90 % of the riverine NO₃^-, indicating the high NO₃^- backgrounds. The release of soil NO₃^- to the river was "transport-limited" in both seasons, i.e., the NO₃^- production/stock in the soils exceeded the capacity of hydrological NO₃^- leaching. In summer, this regime and the NO₃^--plentiful conditions in the soils associated with the strong NO₃^- nitrification led to the high riverine NO₃^- levels. While the in-soil nitrification was weak in winter, the leaching of legacy NO₃^- resulted in the consistently high NO₃^- levels. This study provides insights into the reasons for high NO₃^- levels in pristine or minimally-disturbed rivers worldwide and highlights the necessity to consider NO₃^- backgrounds when evaluating anthropogenic NO₃^- pollution in rivers.